国立研究開発法人 国際農林水産業研究センター | JIRCAS

Diet-tissue Stable Isotopic Fractionation of Tropical Sea Cucumber, <I>Holothuria scabra</I>

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

To provide a basis for a stable carbon and nitrogen isotope ratio (δ13C / δ15N) analysis to determine the assimilated organic matter in sea cucumber, Holothuria scabra, diet-tissue fractionations were experimentally determined by mono-feeding rearing with diatom. While δ15N fractionation of the whole body wall (2.4‰) was similar to the commonly accepted value (2.6 - 4‰), δ13C fractionation of the body wall (4.2‰) showed considerable discrepancy with the commonly accepted value (0 - 1‰) due to the high content (35% dry wt/wt) of calcareous spicules (CaCO3) in the body wall, which had significantly higher δ13C (-8.6‰) than the organic fractions. Computational elimination of spicules based upon spicule content and spicule δ13C reduced the δ13C fractionation of the body wall to 1.5‰, close to the common value. δ13C fractionation after spicule removal by acid decarbonation and subsequent rinsing (3.2‰) did not agree with the common value, and δ15N fractionation was significantly elevated by decarbonation. δ15N and δ13C fractionations of the intestine (1.5 and 2.2‰, respectively) did not agree with the common values. Since δ13C and δ15N of the feces did not differ significantly from those of the diet, feces may be used to determine ingested organic matter in the wild.

刊行年月2013-01-01
作成者WATANABE SatoshiKODAMA MasashiSUMBING Joemel GentelizoLEBATA-RAMOS Maria Junemie Hazel
著者キーワード

calcium carbonate

C/N ratio

spicule

公開者Japan International Research Center for Agricultural Sciences
データ作成日2013-01-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
47
1
開始ページ127
終了ページ134
DOI10.6090/jarq.47.127
権利Japan International Research Center for Agricultural Sciences
言語eng