Cellulase Activity in Blood Cockle (Anadara granosa) in the Matang Mangrove Forest Reserve, Malaysia

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
46-04-10.pdf516.11 KB

To validate the utilization of organic matter, mainly composed of cellulose derived from mangrove trees by bivalves, we analyzed the cellulase activity in blood cockles (Anadara granosa) in the Mantang Mangrove Forest Reserve, Malaysia. In an agar plate assay, unstained circles due to cellulase activity were clearly detected in the extract of blood cockle digestive organs. Additionally, SDS-PAGE zymographic analysis revealed intensive active bands at 9.5 kDa and weak smear bands above 24.5 kDa. These results clearly showed that the blood cockle, the dominant mangrove bivalve species, possesses cellulase activity used to break down cellulose derived from mangrove trees. The present paper first reports the occurrence of the cellulose breakdown function in a mangrove bivalve, blood cockle, via biochemical analyses and suggests the possible ecological function of this species in the mangrove food chain.

刊行年月日
作成者 NIIYAMA Takatoshi TOYOHARA Haruhiko TANAKA Katsuhisa
著者キーワード

agar plate assay

cellulose

digestive organs

zymography

公開者 Japan International Research Center for Agricultural Sciences
データ作成日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
46
4
開始ページ 355
終了ページ 359
DOI 10.6090/jarq.46.355
権利 Japan International Research Center for Agricultural Sciences
言語 eng