国立研究開発法人 国際農林水産業研究センター | JIRCAS

Evaluation of Anti-inflammatory and Anti-allergic Effects of Food Components Using DNA Microarray Analysis

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Many people suffer from allergies, such as asthma, pollinosis and atopic dermatitis, as well as inflammatory diseases. To elucidate the anti-allergic and anti-inflammatory effects of food components, we developed a DNA microarray carrying genes related to immunity, inflammation and housekeeping. Results of gene expression analysis using the DNA microarray showed good reproducibility and correlated well with those of quantitative RT-PCR analysis. DNA microarray analysis showed that the butanol soluble fraction of bitter gourd placenta extract suppressed bacterial lipopolysaccharide (LPS)-induced inflammatory gene expression in mouse macrophage-like cells (RAW264.7). DNA microarray analysis demonstrated that the flavonoid fisetin suppressed the activated T cell membrane induction of gene expression in HMC-1 human mast cells. Thus, we demonstrated the anti-inflammatory effect of bitter gourd and anti-allergic effect of fisetin using DNA microarray analysis. Furthermore, DNA microarray analysis detected gene expression associated with skin irritation using a reconstructed human epidermal model.

刊行年月2010-07-01
作成者KOBORI MasukoNAGAI KanjiTAKAHASHI YumikoOIKE Hideaki
著者キーワード

allergy

bitter gourd

fisetin

inflammation

mast cells

公開者Japan International Research Center for Agricultural Sciences
データ作成日2010-07-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
44
3
開始ページ243
終了ページ248
DOI10.6090/jarq.44.243
権利Japan International Research Center for Agricultural Sciences
言語eng