Deletion Mutations of the Self-incompatibility (S) Locus Induced by Gamma Irradiation in a Wild Diploid Species of Sweet Potato, Ipomoea trifida

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
44-02-04.pdf505.07 KB

Gamma irradiation was used to induce mutations affecting self-incompatibility (SI) in diploid Ipomoea trifida. Plants (S1 homozygote) with developing flower buds were exposed with gamma rays of 10 and 20 Gy. Mature pollen from the irradiated plants was used for crosses with S3 and S10 homozygotes. By pollination test and Southern blot analysis of the M1 progenies, we screened 2 mutant plants carrying a deletion of the S locus derived from irradiated pollen, designated ΔS1. In both mutant alleles, the deleted region was found to exceed a genetic distance of 12 cM around the S locus. Progeny test of both mutant plants showed that neither female nor male gametes carrying the ΔS1 allele were fertile probably due to the lethal effect of the chromosomal deletion spanning the S locus.

作成者 KAKEDA Katsuyuki TSUTSUMI Makiko KOWYAMA Yasuo


S gene

sporophytic self-incompatibility

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 127
終了ページ 131
DOI 10.6090/jarq.44.127
権利 Japan International Research Center for Agricultural Sciences
言語 eng