A Chromatographic Method for Separating and Identifying Intact 4-Methylthio-3-Butenyl Glucosinolate in Japanese Radish (<I>Raphanus sativus</I> L.)
To isolate and measure intact 4-methylthio-3-butenyl glucosinolate (4MTB-GSL) in Japanese radish, we applied a paired ion chromatography method that uses tetradecylammonium ions (TDA) as counter ions. 4MTB-GSL was isolated as ammonium salts from the root of a Japanese radish and identified on the basis of myrosinase treatment, aglycon detection, negative fast atom bombardment (FAB)-mass spectra, and a UV spectrum. The linearity of the prepared standard solution and recovery of that added to Japanese radish extracts were high.
|作成者||ITO HidekazuHORIE Hideki|
|公開者||Japan International Research Center for Agricultural Sciences|
|権利||Japan International Research Center for Agricultural Sciences|