Fitness Characters in Parasexual Recombinants of the Rice Blast Fungus, Pyricularia oryzae
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
We examined the fitness characters in parasexual recombinants derived from cocultures of two isolates, NAO-02 (race 133.1) and TH77-1 (race 047.0), of the rice blast fungus, Pyricularia oryzae, in liquid yeast extract medium. The cocultured isolates were transferred to oatmeal agar to produce a conidial inoculum. Then, the conidial suspension was sprayed on the japonica rice cultivar Akiyutaka, which has two resistance genes to rice blast: Pik and Piz. Three pathogenic variants were isolated from the typical leaf blast lesions that were subsequently produced. We used randomly amplified polymorphic DNA (RAPD) to examine whether the variants were derived from parasexual recombination between the parents. Eleven RAPDs indicated that the variants possessed genomic DNA from both parents. After inoculating Akiyutaka 7 times in succession, the variants maintained their original pathogenicity. The parasexual recombinants produced more conidia and larger lesions on the host than did parent TH77-1, but fewer conidia and smaller lesions than parent NAO-02. Disease development on plants inoculated with either variant or a parental isolate was also midway between the disease caused by each of the parents in a field containing a single cultivar, but the variant caused the most severe disease in a plot with a mixture of cultivars. These results suggest that parasexual recombination produces variation in fitness.
刊行年月日 | |
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作成者 | NOGUCHI Masako Tsujimoto YASUDA Nobuko FUJITA Yoshikatsu |
著者キーワード |
aggressiveness Magnaporthe oryzae |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 41 |
号 | 2 |
開始ページ | 123 |
終了ページ | 131 |
DOI | 10.6090/jarq.41.123 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |