Studies on Speciation in Genus Lycoris Using Interspecific Hybrids and Selfed Plants Produced through Embryo Rescue

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
40-04-04.pdf462.43 KB

This study was carried out to overcome the problems that impede genetic improvement of Lycoris. An improved ovule culture method was developed to rescue the abortive embryo in order to overcome the poor fertility in hybridization of Lycoris. Using this method, a large number of selfed and crossed plants in several species were produced, and the interspecific hybrids which used L. incarnata (sterile) as a cross parent were obtained for the first time. Cytological studies of the S1 plants indicated that polyploidization and aneuploid reduction caused by self-pollination should be among the major factors affecting karyotype evolution in some Lycoris species. Allozyme segregations in polymorphic loci of APT, GOT and EST were observed in S1 of five diploid species, suggesting that each of the species is originally heterozygous. The investigation using genomic in situ hybridization (GISH) was done to clarify the chromosome constitution in Lycoris. The distinction between M + T and A type chromosomes at the DNA sequence level demonstrated that genome differentiation has occurred in the genus Lycoris.

作成者 TARUMOTO Isao MA Biao OGAWA Takeshi



genomic in situ hybridization (GISH)


ovule culture


公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 317
終了ページ 326
DOI 10.6090/jarq.40.317
権利 Japan International Research Center for Agricultural Sciences
言語 eng