国立研究開発法人 国際農林水産業研究センター | JIRCAS

Skim Milk Drastically Improves the Efficacy of DNA Extraction from Andisol, a Volcanic Ash Soil

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

There is an increasing interest in the extraction of DNA from soil, since molecular techniques facilitate the analysis of the microbial community including unculturable microorganisms. Though various methods of direct DNA extraction have been devised, it still remains difficult to extract DNA from some soils. We developed an improved method to extract DNA from such “recalcitrant soils”. Andisol, volcanic ash soils are recalcitrant in this context. Skim milk increased the efficacy of DNA extraction from such soils, when added to the extraction buffer as an adsorption competitor to soil particles. This method is applicable to molecular community analysis of soils which strongly adsorb DNA.

刊行年月2005-10-01
作成者TAKADA HOSHINO YukoMATSUMOTO Naoyuki
著者キーワード

environmental DNA

microbial community analysis

molecular methods

unculturable microorganisms

公開者Japan International Research Center for Agricultural Sciences
データ作成日2005-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
39
4
開始ページ247
終了ページ252
DOI10.6090/jarq.39.247
権利Japan International Research Center for Agricultural Sciences
言語eng