Evaluation of Preservation Techniques of Microorganism Resources in the MAFF Genebank
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
In the central bank of the microorganisms section in the Ministry of Agriculture, Forestry and Fisheries (MAFF) Genebank in Tsukuba, 14,836 strains of fungi, bacteria, yeasts and others are preserved by various methods including cryopreservation and freeze-drying. To evaluate preservation techniques for these microorganisms, the viability of the preserved microorganisms was examined at regular intervals. Almost all tested strains, except for 5 strains out of 2,334 strains, of yeasts, bacteria and Actinomycetes were preserved well in freeze-dried form, indicating that freeze-drying is suitable for their preservation. As for fungi and Oomycetes, 6,578 strains out of 6,681 tested strains (98.5%) and 264 (63.8%) of 414, respectively, survived one-year preservation in the vapor phase of liquid nitrogen. The details on survival of the preserved fungal strains were as follows: 99.0% (1,107 strains/1,118 tested strains) survival in Ascomycota, 96.1% (1,552/1,615) in Basidiomycota, 98.6% (73/74) in Zygomycota, and 99.3% (3,846/3,874) in anamorphic fungi. Thus, cryopreservation is excellent for preservation of most fungus strains, but is not suitable for preservation of many Oomycetous strains.
刊行年月日 | |
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作成者 | NAGAI Toshirou TOMIOKA Keisuke TAKEUCHI Kasumi IIDA Motoko KAWADA Masae SATO Toyozo |
著者キーワード |
cryopreservation culture collection freeze-drying |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 39 |
号 | 1 |
開始ページ | 19 |
終了ページ | 27 |
DOI | 10.6090/jarq.39.19 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |