Respiration rate is the necessary parameter for designing the storage conditions. In the present investigation, the effects of storage temperatures (5, 10, 15, 20, 25℃) and gas composition on the O₂ consumption and the CO₂ production have been studied by using the flow through system. The ratio of the respiration rate under controlled atmosphere (CA) conditions (1.6 to 20.7% O₂, 0.2 to 10.2% CO₂, balanced with N₂) were calculated against the values under normal air condition. As a result, it was found that a decrease in O₂ concentration suppressed O₂ uptake at each temperature involving an increase of the respiratory quotient. On the other hand, an increase in CO₂ concentration up to 10% suppressed both O₂ uptake and CO₂ output at 5-15℃ with almost a constant respiratory quotient. Thus, low temperature combined with CA having 5-10% CO₂ will be effective to suppress the respiration rate of tree-ripe ‘Irwin' mango fruit, and O₂ should be higher than 5% to avoid anaerobic respiration.