Discrimination among Japanese Species of the Orius Flower Bugs (Heteroptera: Anthocoridae) Based on PCR-RFLP of the Nuclear and Mitochondrial DNAs

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

A PCR-RFLP-based method of species identification was considered for 5 Japanese species of Orius Wolff flower bugs, Orius strigicollis (Poppius), O. minutus (Linnaeus), O. sauteri (Poppius), O. nagaii Yasunaga, and O. tantillus (Motschulsky). Nucleotide sequences of the internal transcribed spacer 1 of the nuclear ribosomal gene and a portion of mitochondrial cytochrome oxidase subunit I gene were compared among species and recognition sites of diagnostically useful restriction enzymes were examined. The PCR-RFLP analysis using 108 individuals of 26 laboratory strains confirmed that the 5 species could be correctly identified by banding patterns generated using these DNA regions. Because our PCR primers can amplify DNA fragments from DNA template extracted from both freshly killed insects and dried specimens stored at room conditions, the PCR-RFLP-based method was considered useful for analyses of field populations in which researchers store and accumulate field-collected samples before they perform laboratory examinations.

刊行年月日
作成者 MURAJI Masahiko KAWASAKI Kenjiro SHIMIZU Toru NODA Takashi
著者キーワード

natural enemy

predatory bugs

species identification

internal transcribed spacer 1

cytochrome oxidase subunit I

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
38
2
開始ページ 91
終了ページ 95
DOI 10.6090/jarq.38.91
権利 Japan International Research Center for Agricultural Sciences
言語 eng

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