Agrobacterium-Mediated Transformation in Liliaceous Ornamental Plants

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
36-03-01.pdf724.2 KB

Studies on Agrobacterium-mediated transformation in 3 Liliaceous ornamental plants, Lilium formosanum, Agapanthus praecox ssp. orientalis and Muscari armeniacum, were described. Three different strains of A. tumefaciens were used, all of which harbored the binary vector carrying the nptII, hpt and gus-intron genes in the T-DNA region. For L. formosanum, no transgenic tissues nor plants were obtained after co-cultivation of organogenic calli with A. tumefaciens, although transient expression of the gus gene could be detected in the calli during co-cultivation. On the other hand, several hygromycin-resistant (Hygr) cell clusters were obtained for both A. praecox ssp. orientalis and M. armeniacum following the transfer of co-cultivated embryogenic calli onto hygromycin (Hyg)-containing media. Hygr calli developed into complete plants via somatic embryogenesis, and most of them were confirmed to be transgenic plants based on GUS histochemical assay and PCR analysis. Southern blot analysis revealed the integration of 1 to 5 copies of the transgene into the genome of the transgenic plants of both 2 species, but most of them had 1 or 2 copies. Agrobacterium-mediated transformation systems developed for A. praecox ssp. orientalis and M. armeniacum may be useful as a tool for their genetic improvement as well as molecular biology studies.

作成者 SUZUKI Sakae NAKANO Masaru


embryogenic callus



transgenic plant

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 119
終了ページ 127
DOI 10.6090/jarq.36.119
権利 Japan International Research Center for Agricultural Sciences
言語 eng