国立研究開発法人 国際農林水産業研究センター | JIRCAS

Specific Amplification of Microsporidian DNA Fragments Using Multiprimer PCR

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Using a mixture of several primers, the suitability of multiprimer PCR for the early and simultaneous detection of various kinds of infectious microsporidia of silkworms was evaluated. As a result, specific DNA sequences were amplified by PCR using several primers designed for this study only when genomic DNA of the target microsporidia was used as the DNA template. However, no PCR products were obtained when genomic DNA of the silkworms or other microorganisms was used as the DNA template. In addition, specific DNA sequences were amplified by multiprimer PCR even when silkworms were infected with various kinds of microsporidia. When genomic DNA extracted from silkworm eggs infected with Nosema bombycis was used as the DNA template, the specific DNA sequences were amplified by multiprimer PCR. In addition, similar results were obtained even when genomic DNA extracted from silkworms infected with N. bombycis was used as the DNA template. These findings suggest that multiprimer PCR using several primers designed for this study is suitable for pebrine inspection of silkworm eggs.

刊行年月2002-04-01
作成者HATAKEYAMA YoshinoriHAYASAKA Shoji
著者キーワード

Nosema

Bombyx mori

Silkworm pebrine

transovum transmission

公開者Japan International Research Center for Agricultural Sciences
データ作成日2002-04-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
36
2
開始ページ97
終了ページ102
DOI10.6090/jarq.36.97
権利Japan International Research Center for Agricultural Sciences
言語eng