国立研究開発法人 国際農林水産業研究センター | JIRCAS

Fundamental Studies on Freezing Hardiness and Wet Tolerance in Italian Ryegrass (<i>Lolium multiflorum</i> Lam.) Breeding

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

An artificial screening technique based on lethal temperature for 50% kill of plants (LT50) was developed to evaluate the freezing hardiness in ltalian ryegrass (Lolium multiflorum Lam.) breeding programs. This technique was found to be suitable for evaluating the changes in freezing hardiness in natural and artificial environments, and the freezing hardiness of ltalian ryegrass was enhanced through this screening method. To elucidate the mechanisms of freezing hardiness, it was found that the N-terminal amino acid sequence of a protein induced by hardening was similar to the partial amino acid sequence of the abscisic acid (ABA)-inducible protein of alfalfa. ABA thus appeared to be involved in the induction of freezing hardiness in the hardening process. An artificial screening technique based on rooting on the flooded soil surface was also developed to evaluate the wet tolerance. The enhancement of the wet tolerance was achieved through selection using this artificial screening technique. To elucidate the mechanisms of wet tolerance, it was shown that aerenchyma formation in roots under flooding conditions was closely associated with the wet tolerance. lncrease in the number of roots with the development of the aerenchyma thus appeared to enhance the wet tolerance. Analysis of the alcohol dehydrogenase (ADH) isozyme and protein synthesis patterns indicated that anaerobic metabolism was possibly induced in roots under flooding conditions.

刊行年月2002-01-01
作成者TASE Kazuhiro
著者キーワード

screening technique

amino acid sequence

two-dimensional polyacrylamide gel electrophoresis

公開者Japan International Research Center for Agricultural Sciences
データ作成日2002-01-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
36
1
開始ページ7
終了ページ16
DOI10.6090/jarq.36.1
権利Japan International Research Center for Agricultural Sciences
言語eng