国立研究開発法人 国際農林水産業研究センター | JIRCAS

Effects of Medium Conditions on Adventitious Bud Formation in Immature Mulberry Leaves

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Immature mulberry leaves isolated from winter buds were cultured on MS media7) differing in the nitrogen source, sugar(s) or pH, or containing various kinds and concentrations of plant hormones, antibiotics or a herbicide. The ratio of the concentration of nitrate ion to that of ammonium ion remarkably affected the frequency of adventitious bud formation (FABF) and ratios in the ranges of 1:1 and 3:1 were optimum. The most suitable sugars for adventitious bud formation were sucrose, glucose and fructose among the tested sugars. The highest FABF was usually observed in the medium containing 1 μM thidiazuron and the addition of 1 μM abscisic acid further enhanced FABF. Changes of pH and addition of other plant hormones did not produce better results than in the control. Kanamycin, geneticin, hygromycin and bialaphos suppressed adventitious bud formation, showing that the antibiotics and the herbicide may be effective for the selection of transgenic plantlets of mulberry.

刊行年月1999-10-01
作成者Hiroaki YAMANOUCHI Akio KOYAMA Hiroaki MACHII
データ作成日1999-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
33
4
開始ページ267
終了ページ274
言語eng