Structural Changes of Forage Grass Lignin by Rumen Digestion : Characteristics of Soluble Lignin Released from Timothy (Phleum pratense L.) by In Vitro Rumen Digestion

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

Timothy, harvested at the seed setting stage, was subjected to 3 different treatments: (1) ball milling followed by in vitro rumen digestion, (2) ball milling followed by cellulase digestion, (3) digestion by a heifer, and then dioxane-soluble lignins (RDL, CDL and HDL, respectively) were isolated from the 3 resultant residues. RDL contained a larger amount of lignin components and smaller amounts of carbohydrate residues, and bound phenolic acids than CDL and its composition resembled that of HDL. Alkaline nitrobenzene oxidation and IR spectra showed that all the lignins consisted of guaiacyl-syringyl lignins. The molar ratio of syringaldehyde to vanillin was higher for HDL than for CDL and RDL. In gel permeation chromatograms, RDL and HDL were distributed in lower molecular weight regions than CDL. The results suggested that timothy lignin was depolymerized and the phenolic acids bound to timothy lignin disappeared by rumen fermentation.

作成者 Tsuneo KONDO Tomoko OHSHITA Tadashi KYUMA
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 49
終了ページ 53
言語 eng