国立研究開発法人 国際農林水産業研究センター | JIRCAS

Identification of mRNAs Differentially Expressed between Embryogenic and Non-Embryogenic Cultivars of Eggplant during Somatic Embryogenesis

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

The molecular basis of differential cultivar response to the induction of somatic embryogenesis was investigated by using the digoxigenin (DIG)-differential display with 2 eggplant (Solanum melongena L.) cultivars, Wase Shinkuro (WS) and Kumamoto Naga (KN). WS produced embryogenic callus (EC) which was capable of differentiating into mature somatic embryos while KN cultures treated similarly failed to differentiate somatic embryos. DIG-differential display of 0, 1, 2, 4, and 10-day old cultures of both cultivars revealed several PCR products derived from WS, but not from KN, at a crucial stage during embryogenesis. These results indicated that the cultivar difference in EC induction may be attributed, in part, to differences in mRNA expression between the cultivars during the culture process.

刊行年月1996-07-01
作成者John Cliff AFELE Yutaka TABEI Tomohiro YAMADA Takahiro MOMIYAMA Fumio TAKAIWA Toshiaki KAYANO Shigeo NISHIMURA Takeshi NISHIO
データ作成日1996-07-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
30
3
開始ページ175
終了ページ179
言語eng