Production and Specificity of Monoclonal Antibodies Reactive to Progesterone

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

Monoclonal antibodies to progesterone 11α-hydroxy hemisuccinate were prepared. Supernatant of clone hybridoma reacted positively to the progesterone solution. Dotblot analysis revealed a single precipitation band when rabbit anti-mouse IgM and anti-mouse κ were used. These monoclonal antibodies did not react with pregnenolone, testosterone, estrone, estradiol-17β, aldosterone, hydrocortisone (cortisol), cortisone, corticosterone and 11α-dehydroxycortisone (DOC). Radioimmunoassay using those monoclonal antibodies was developed. Data from this assay supported the following conclusions: (1) association constant (KA) of supernatant of clone PrM for progesterone was 2×1010, (2) monoclonal antibodies reacted to progesterone most efficiently at 1 : 10,000 dilution, and (3) efficiency of reactivity of monoclonal antibodies was higher than that of rabbit antibodies.

刊行年月日
作成者 Chung-Boo KANG Ikuo DOMEKI Hideo KAMOMAE Toshio IKEDA Masayoshi YUKAWA Takashi ONODERA
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
28
2
開始ページ 150
終了ページ 153
言語 eng

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