In Vitro Propagation Techniques of Tea Plants

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

Effective methods, simple and reliable of in vitro propagation of tea plants have successfully been established. The growth of shoots in culture of shoot tips and axillary buds was remarkably accelerated when the media were mixed with the combination of BA(0.1-1.0 mg/l) + GA3(5.0 mg/l) or BA(1.0, 5.0 mg/l) + GA3(1.0 mg/l). And it was observed that the axillary bud culture provided a more effective method, which was easier, simpler and quicker in securing the growth of shoots, as compared with the shoot tip culture. Differentiation of organs was observed on adventitious embryos (cotyledons used as the explants) and adventitious buds (stem segments used as the explants) cultured on the media containing BA(1.0-5.0 mg/l) and IAA(0.01-1.0 mg/l) + GA3(1.0-5.0 mg/l), respectively. A small shoot each developed from these differentiated adventitious embryos and buds on the same media. As the number of shoots grown was limited, passage culture by in vitro cutting of nodal segment culture with axillary buds was repeated to propagate them, for which the optimum combinations of hormones were IBA(0.1 mg/l) + BA(1.0 mg/l) + GA3(5.0 mg/l) or IBA(0.1 mg/l) + BA(0.1 mg/l) + 2iP(5.0mg/l) + GA3(5.0 mg/l). By repeating this technique of nodal segment culture every 2 months, at least 47,000 (i.e. 66) plantlets could be multiplied from an original plantlet in a year. And rooting from these shoots was easily induced on 1/2 or 1/4 MS media incorporated with IBA(0.5-1.0 mg/l).

作成者 Yoriyuki NAKAMURA
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 185
終了ページ 194
言語 eng