Production of Somatic Hybrid Plants through Protoplast Fusion in Citrus
JARQ : Japan Agricultural Research Quarterly
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
本文フルテキスト
22-3-181-188.pdf1.24 MB
Somatic hybrid plants were obtained by protoplast fusion in the Rutaceae family. Protoplasts isolated from nucellar calli and from leaves of nucellar seedlings were fused by the PEG method. The fusion products were cultured in a Murashige and Tucker medium containing 0.6 M sucrose. In this medium, some colonies developed into the whole plants through embryogenesis. Almost all of the plants were shown to be somatic hybrid, which were proven by the restriction endonuclease analysis of nuclear ribosomal DNA. The chromosome number of the hybrid plants was 36, which was the sum of the parents (2n=18).
刊行年月日 | |
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作成者 | SHOZO KOBAYASHI TOSHIFUMI OHGAWARA |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 22 |
号 | 3 |
開始ページ | 181 |
終了ページ | 188 |
言語 | eng |