Characterization of Leaf Transcriptome in a Tropical Tree Species, Shorea curtisii, over a Flowering Season

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

General flowering (GF) is a synchronous flowering phenomenon in the Southeast Asian tropical rainforests that occurs at irregular intervals of multiple years. While in this study, the leaf transcriptome of a GF species, Shorea curtisii obtained from three-time points – before and after floral initiation, and post-flowering stage – was sequenced, the unpredictable intervals of GF raise conservation concerns for these under-researched forests with rich economically and ecologically important species. We assembled 243,759,478 sequencing reads into 39,943 non-redundant unigenes including 677 putative homologs of Arabidopsis thaliana flowering-related genes. Differential expression analysis conducted on pairwise comparisons of the time points identified 930 differentially expressed unigenes, which includes 17 flowering-related homologs. The differential expression of unigenes with significant enrichments of functions related to drought corroborated the involvement of drought as an environmental cue for GF, with the outcomes of this study offering an insight into the conservation of floral regulatory genes and pathways in Shorea and possibly being used as a model to better understand the floral initiation cues and regulation of GF trees.

作成者 Ahmad Husaini SUHAIMI Masaki J. KOBAYASHI Akiko SATAKE Soon Leong LEE Norwati MUHAMMAD Tatsuya OTANI Toshiaki KONDO Naoki TANI Suat Hui YEOH
著者キーワード Dipterocarpaceae flowering time mass flowering next-generation sequencing transcriptomics
公開者 Japan International Research Center for Agricultural Sciences
受付日 2022-04-12
受理日 2022-07-19
開始ページ 139
終了ページ 144
DOI 10.6090/jarq.57.139
言語 eng