Comparison of Carotenoid Accumulation and Abscisic Acid Content in Fruit-color Altered Mutant and Original Types in Citrus

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
Carotenoid mutants are useful for elucidating carotenoid metabolism. ‘Kusaie’ is a yellow-fruited form of ‘Rangpur’ (Citrus limonia Osbeck). Hyuganatsu (C. tamurana hort. ex Tanaka) is a medium-sized, light yellow fruit. Its bud mutation, wherein the fruit rind color becomes orange, is named ‘Orange-hyuga.’ To elucidate the mechanism underlying this alteration, their carotenoid composition and abscisic acid content were analyzed. In the flavedo of ‘Kusaie,’ significant phytoene accumulation, decreased xanthophyll, and ABA contents were observed, suggesting that phytoene desaturase was suppressed. Moreover, the total carotenoids in the juice sac of ‘Kusaie’ was significantly lesser than those in that of ‘Rangpur,’ suggesting that the production of carotenoids is suppressed in ‘Kusaie.’ In ‘Orange-hyuga,’ the total carotenoids was approximately 6-fold that of Hyuganatsu, and a significant xanthophyll accumulation, especially violaxanthin, was observed. Furthermore, ABA content was significantly lower. Accordingly, those might be associated with an altered gene expression balance upstream and downstream of biosynthesis. Difference in biosynthesis regulation in these color-altered cultivars might cause a change in the carotenoid contents and composition in fruits, which seemed to result in color changes from reddish-orange in ‘Rangpur’ to yellow in ‘Kusaie’ and from a light yellow color in Hyuganatsu to an orange color in ‘Orange-hyuga.’ In this study, these color-changing citrus mutants were observed to accumulate carotenoids differently from the original type, and we speculated that different changes occurred in carotenoid biosynthesis regulation.
作成者 Naoko NAKAJIMA Yoshinori IKOMA Hikaru MATSUMOTO Masaya KATO Takeshi KUNIGA
著者キーワード color-changing mutants Hyuganatsu ‘Rangpur’ ‘Orange-hyuga’ ‘Kusaie’
公開者 Japan International Research Center for Agricultural Sciences
受付日 2020-10-14
受理日 2020-12-25
開始ページ 359
終了ページ 366
DOI 10.6090/jarq.55.359
言語 eng