Evaluation of an Enzyme-Linked Immunosorbent Assay(ELISA) Using Mycobacterium phlei - Absorbed Serum for the Diagnosis of Bovine Paratuberculosis in a Field Study

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

 The specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paratuberculous cattle was improved by absorbing the bovine sera with a suspension of Mycobacterium phlei-killed organisms. The newly developed ELISA technique was evaluated and compared with the complement fixation (CF) test for the detection of antibodies to Mycobacterium paratuberculosis. Thirty-eight (66%) of 58 fecal culture positive cattle in a M. paratuberculosis infected herd were positive in the ELISA test, whereas the CF test yielded only 15 (26%) positive results. Calves experimentally infected with M. paratuberculosis seroconverted in the ELISA test at the earlier stage of infection than in the CF test. The ELISA was proposed as an alternative to the CF test for the detection of antibodies against M. paratuberculosis in bovine serum. Application of the ELISA as a diagnostic test for subclinical infection of cattle with M. paratuberculosis was proved to be very useful.

刊行年月日
作成者 YUICHI YOKOMIZO
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
20
1
開始ページ 60
終了ページ 67
言語 eng

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