国立研究開発法人 国際農林水産業研究センター | JIRCAS

Genetic Differentiation of Asian Cultivated Rice as Revealed by RFLP Analysis

JIRCAS international symposium series
ISSN
13406108
書誌レコードID(総合目録DB)
AA1100908X
本文フルテキスト
Restriction Fragment Length Polymorphism (RFLP) was studied in 135 accessions of Asian cultivated rice, Oryza sativa L., of which 123 were landraces collected mainly from Asia. For Southern hybridization, 56 clones of rice genomic library and one maize cDNA clone were selected as DNA probes. Various kinds of polymorphism were detected, and almost all the cultivars could be distinguished from each other. Cluster analysis (CA) and principal component analysis (PCA) applied to the RFLP data revealed two major groups of landraces, with only a few cultivars intermediate between the groups. The groups, designated as clusters I and II based on the cluster analysis, were considered to correspond with the conventional varietal groups (lndica and Japonica), because they showed differences in phenol color reactions, Est 3 genotypes and responses to low temperature. Cluster I contained the cultivars collected from Southeast Asia, South Asia and their vicinity, while cluster II consisted of those from Southeast Asia and the temperate zone of Asia. Their distribution areas overlapped in Southeastern Asia. Several minor groups were recognized in each major group. Most of the smaller groups showed a particular geographical distribution, indicating that indigenous landraces had evolved in different areas over a long period of time.

作成者Makoto KAWASE
公開者Japan International Research Center for Agricultural Sciences
データ作成日1993-08-25
2
開始ページ81
終了ページ87
言語eng