Toward the Future of Agriculture


  • お知らせ


  • 2020/2/10

    Our paper has been published on Sorgoleone release from sorghum roots shapes the composition of nitrifying populations, total bacteria, and archaea and determines the level of nitrification.

Sarr et al. Biology and Fertility of Soils 56, 145–166 (2020)

  • 2019/07/29
    • Our BNI-friend Tim Searchinger's report on "Creating Sustainable Food Future" published

BNI is mentioned on page 11 and 48 on 'SYNTHESIS REPORT'


Di, T. et al. 2018. Plant and Soil 423: 99-110.

Title:  Further insights into underlying mechanisms for the release of biological nitrification inhibitors from sorghum roots.

Conclusion: Rhizosphere pH has a major influence on hydrophilic-BNIs release, but not on the release of hydrophobic-BNIs. The low rhizosphere pH stimulated PM-H+ ATPase activity; H+-extrusion is closely coupled with hydrophilic-BNIs release. Anion-channel blockers stimulated H+ extrusion and hydrophilic-BNIs release. Our results indicate that some unknown membrane transporters are operating the release of protonated BNIs, which may compensate for charge balance when transport of other anions is suppressed using anion-channel blockers. A new hypothesis is proposed for the release of hydrophilic-BNIs from sorghum roots.

Subbarao, G. V. et al. 2017. Plant Science 262: 165-168.

Title:  Genetic mitigation strategies to tackle agricultural GHG emissions: The case for biological nitrification inhibition technology. 


  • Transformative biological technologies need to be developed to reduce nitrification in agricultural systems.
  • Genetic mitigation could be one such option where next-generation of crop varieties need to be bred by incorporating traits such as BNI.
  • Low-nitrifying production systems to be developed using BNI-enabled crops and pastures.

Zeng, H. et al. 2016. Plant and Soil, 398, 301-312.

Title: Transcriptional response of plasma membrane H+-ATPase genes to ammonium nutrition and its functional link to the release of biological nitrification inhibitors from sorghum roots

Results: Results suggest that the functional link between PM H+-ATPase activity and BNIs release is evident only at NH4+ levels of ≤1.0 mM in the rhizosphere. The variation in PM H+-ATPase activity by NH4+ is due to transcriptional regulation of five isoforms of the H+-ATPases. The stimulatory effect of NH4+ on BNIs release is functionally associated with NH4+ assimilation and not just with NH4+ uptake alone.