High-Impact Research

International differential system to protect against rice blast disease

Description

Blast disease has been found to have caused serious damage to rice production in all areas of the world where rice is cultivated, from tropical to temperate regions. The use of resistance varieties is the most economically and efficient way to protect against rice blast disease in developing countries. The differential system, which can clarify the pathogenicity of blast isolates and resistance in rice cultivars, is a basic and important tool for breeding and pathological works. However, few countries or research institutes have used the differential system and applied the developed technology for crop protection. 
To establish this protection system against blast disease in developing countries and regions, JIRCAS has conducted  international collaborative research for developing and distributing the differential system in Asian and African regions. 
Under the international research network, blast isolates and rice germplasm were collected. The pathogenicity of blast isolates using international differential varieties (DVs) and genetic variation of resistance in resistant rice cultivars were clarified. Based on these information, international standard differential blast isolates (ISDBIs) collected from different origins under the research network were selected (Table 1). 
The ISDBIs and DVs comprise the international differential system, and the system is being used as the international standard for characterizing the pathogenicity of blast isolates and resistance of rice cultivars. It can also be applied to breeding works in rice cultivars and pathological studies for the development of a protection system.
Participants of the research network including Indonesia, Philippines, Vietnam, Laos, and Bangladesh also selected their respective local standard differential blast isolates, and these domestic differential systems were applied to breeding and pathological studies in each country.
One of the network’s research achievements was the clarification of the wide variations in blast races (Fig. 1). The frequency of blast isolates virulent to DVs was clarified in each country and region and at the global level. The frequency of virulent blast isolates varied among DVs and regions. Particularly, high frequencies of wide-spectrum blast isolate virulence to DVs were found in Bangladesh and West Africa. 
Furthermore, the highest diversities of blast races were found from Yunnan province, China, to Bangladesh. The diversities of blast races were corresponded with the those of resistance in rice cultivars. The relationships between blast races and rice varieties are explained by the gene-for-gene theory. The differential system will make it possible to characterize previous resistance genes and to find new genes, such as partial resistance genes, and provide access to the development of application methods for them in rice breeding. The information, i.e., the differential system and these new approaches, will contribute to the development of a durable protection system and for harmonizing agriculture with environment. 

Figure, table

  1. Table 1. International differential system for rice blast disease

    The international differential system consists of 25 differential varieties and the susceptible variety LTH, and 53 international standard blast isolates (Several blast isolates were omitted in the table). Blast race names were designated to each blast isolate selected according to the method of Hayashi and Fukuta (2009). Reactions: S: virulent (susceptible), M: moderate, R: Avirulent (resistant)

     

  2. Fig. 1. Frequency of virulent blast isolates to differential variety in each country and region 

    Wide variations in virulent blast isolates to each differential variety were found, and they varied among regions. Wide-spectrum blast isolates virulent to differential varieties were found in Bangladesh and West Africa at high frequencies.

     

Affiliation

Japan International Research Center for Agricultural Sciences Tropical Agriculture Research Front

Classification

Research

Research project

“Pest and Disease Control” Development of technologies for the control of migratory plant pests and transboundary diseases

“Food Security in Africa” Development of sustainable technologies to increase agricultural productivity and improve food security in Africa

Program name

Stable Agricultural Production

Term of research

FY2020(FY2016~FY2020)

Responsible researcher

Fukuta Yoshimichi ( Tropical Agriculture Research Front )

KAKEN Researcher No.: 40399374
MIERUKA ID: 001798

SAITO Hiroki ( Tropical Agriculture Research Front )

Obara Mitsuhiro ( Biological Resources and Post-harvest Division )

KAKEN Researcher No.: 10455248
MIERUKA ID: 001771

Yanagihara Seiji ( Biological Resources and Post-harvest Division )

MIERUKA ID: 001780

Hayashi Nagao ( Institute of Agrobiological Sciences, NARO )

KAKEN Researcher No.: 90391557

ほか
Publication, etc.

Kawasaki-Tanaka et al. (2016) Plant Dis., 100:816-823
https://doi.org/10.1094/PDIS-04-15-0442-RE

Khan et al. (2016) Plant Dis., 100:2025-2033
https://doi.org/10.1094/PDIS-12-15-1486-RE

Khan et al. (2017) Breed. Sci., 67:493-499
https://doi.org/10.1270/jsbbs.17039

Odjo et al. (2017) Breed. Sci., 67:500-508
https://doi.org/10.1270/jsbbs.17051

Fukuta et al. (2019) Plant Dis., 103:3181-3188
https://doi.org/10.1094/PDIS-04-19-0870-RE

Fukuta et al. (2019) Breed. Sci., 69:672-679
https://doi.org/10.1270/jsbbs.19065

Nguyen et al. (2020) Plant Dis. 104:381-387
https://doi.org/10.1094/PDIS-05-19-1008-RE

Xangsayasane et al. (2020) Plant Health Prog., 21:248-255
https://doi.org/10.1094/PHP-05-20-0041-RS

Orn et al. (2020) Breeding Science
https://doi.org/10.1270/jsbbs.20052

Santoso et al. Plant Dis. 105:675-683
https://doi.org/10.1094/PDIS-05-20-0949-RE

Japanese PDF

2020_B11_A4_ja.pdf444.1 KB

2020_B11_A3_ja.pdf524.71 KB

English PDF

2020_B11_A4_en.pdf381.92 KB

2020_B11_A3_en.pdf384.18 KB

Poster PDF

2020_B11_poster.pdf713.33 KB

* Affiliation at the time of implementation of the study.