Identification of the fatty acid and the fatty acid methyl ester as the new nitrification inhibitors

Description

The tropical pasture grass, Brachiaria humidicola (Rendle) Schweick, produces nitrification inhibitory compounds (termed biological nitrification inhibitors or BNIs) in its shoot and root tissues, and releases BNIs from its roots. During this study, two BNI compounds were isolated and identified from the shoot tissue of B. humidicola using activity-guided fractionation.

The BNI compounds in the shoot tissue were identified as linoleic acid (LA) and linolenic acid (LN) using authentic chemicals (ED80 16.0 mg ml-1 for both LA and LN) for verification (Fig. 1). None of the other tested free fatty acids namely stearic acid, oleic acid, arachidonic acid, and vaccenic acid showed any inhibitory effects on nitrification. Among the fatty acid methyl esters (FAME) evaluated [methyl oleate, methyl linoleate (LA-ME) and methyl linolenate (LN-ME)], only LA-ME showed any inhibitory effect (ED80 8.0 mg ml-1) (Figs. 1, 2). The inhibitory effect of LA, LN and LA-ME on soil nitrification was stable for 120 days at 20°C (Fig. 3). Soil treated with LA, LN and LA-ME showed a very low accumulation of NO3- and the maintenance of soil inorganic N in the NH<sub>4</sub><sup>+</sup>+ form (Fig. 3). The inhibitory effect of LA-ME on soil nitrification was greater than that of LA, LN or nitrapyrin (commercial nitrification inhibitor) (Fig. 3). Both LA and LN suppressed soil nitrifier activity by blocking AMO (ammonia monooxygenase) and HAO (hydroxylamine oxidoreductase) enzymatic pathways in Nitrosomonas europaea. Commercial nitrification inhibitors (such as nitrapyrin or dicyandiamide (DCD)) are not effective (nitrapyrin is volatile at temperatures >5C, thus is not effective in tropical environments; DCD is highly soluble in water, thus leaches out of fertilizer zone, thus is not effective in field environments) in tropical environments, thus are not adopted for production agriculture in tropics.

Since LA, LN and LA-ME can be produced from vegetable oils such as soybean, flax or sunflower and are more effective and stable (these compounds are bound to the soil thus do not leach out from the point of application to the fertilizer zone, which is in addition to their stability at tropical temperatures) in tropical soils, they have the potential for use as nitrification inhibitors in production agriculture.

Figure, table

  1. Fig. 1. Structure formulae of various fatty acid and fatty acid ester. Substance with an enclosure has nitrification inhibitory activity.

    Fig. 1. Structure formulae of various fatty acid and fatty acid ester. Substance with an enclosure has nitrification inhibitory activity.

  2. Fig. 2. Relative effectiveness of substances in inhibiting Nitrosomonas europaea activity in an in vitro assay.

    Fig. 2. Relative effectiveness of substances in inhibiting Nitrosomonas europaea activity in an in vitro assay.

  3. Fig. 3. Relative stability of the inhibitory effects on soil nitrification from linoleic acid, linolenic acid, methyl linoleate and nitrapyrin during 120-day incubation period at 20°C.

    Fig. 3. Relative stability of the inhibitory effects on soil nitrification from linoleic acid (1,000 µg g-1 soil), linolenic acid (1,000 µg g-1 soil) , methyl linoleate (1,000 µg g-1 soil) and nitrapyrin (4.5 µg g-1 soil) during 120-day incubation period at 20°C.

Affiliation

Japan International Research Center for Agricultural Sciences Crop, Livestock and Environment Division

Classification

Administration A

Research project

Utilization of Biological Nitrification Inhibition (BNI) function for the development of breeding materials and application to cropping systems

Program name

Environment and Natural Resource Management

Term of research

FY 2006-2007 (FY 2006-FY 2010)

Responsible researcher

Subbarao Guntur Venkata ( Crop, Livestock and Environment Division )

KAKEN Researcher No.: 00442723

Nakahara Kazuhiko ( Biological Resources and Post-harvest Division )

KAKEN Researcher No.: 90241778
MIERUKA ID: 001774

Ishikawa Takayuki ( Crop, Livestock and Environment Division )

Yoshihashi Tadashi ( Crop, Livestock and Environment Division )

KAKEN Researcher No.: 60450269
MIERUKA ID: 001766

Ono Hiroshi ( National Food Research Institute )

KAKEN Researcher No.: 90353995

Kameyama Mayumi ( National Food Research Institute )

KAKEN Researcher No.: 80353994

Yoshida Mitsuru ( National Food Research Institute )

KAKEN Researcher No.: 60353992

ほか
Publication, etc.

Subbarao, G. V. et al. (2008) Plant and Soil, 313: 89-99

https://doi.org/ 10.1007/s11104-008-9682-5

Ishikawa, et al. (2012) "Nitrification inhibitor, and soil conditioner and fertilizer containing the same". JP5067520

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