Near-isogenic lines carrying QTL for high spikelet number with the genetic background of IR64, an Indica-type rice variety

Description

IR64, recognized globally as a high quality Indica-type rice variety, was first released by the International Rice Research Institute (IRRI) in 1985. To improve the yield potential of IR64, a set of near-isogenic lines (NILs) of IR64 with increased total spikelet number per panicle (TSN) was developed using New Plant Type (NPT) varieties as tropical Japonica-type donor parents.

A total of five NILs derived from different donor parents were developed through marker-assisted selection. Regardless of the donor parents, Quantitative Trait Locus (QTL) for high TSN was detected on common region of the long arm of chromosome 4 (Table 1, Fig. 1). We designated this QTL as qTSN4. Marker information linked to qTSN4 is shown in Table 1. NILs have 196-239 spikelets per panicle (i.e., 40-70% greater TSN than IR64), which is attributed to the increase in larger number of spikelets on the secondary and ternary rachis branches.  Variation in TSN was observed among NILs; the number of spikelets was greatest in IR64-NIL2 (IR65564-2-2-3 as donor parent) and in IR64-NIL4 (IR66215-44-2-3 as donor parent).

In this study, we succeeded in developing five NILs for high TSN with a rice variety (IR64) genetic background by employing molecular marker-assisted selection. The materials are expected to be useful for enhancing the yield potential of rice varieties. These NILs are also available for understanding the genetic basis of TSN and the effects of single QTL/gene by testing it under different environmental conditions. Further investigation is required to determine if the variation in panicle architecture is simply due to the allelic effects or not.

Figure, table

  1. Fig.1. The location of QTLs for spikelet number per panicle on the long arm of chromosome 4.
  2. Table 1. Marker information on QTLs and panicle structure of IR64 and its NILs for high TSN
  3. Fig.2. Panicle architecture of IR64 and its NILs for increased spikelet number
Affiliation

Japan International Research Center for Agricultural Sciences Biological Resources and Post-harvest Division

Classification

Administration A

Research project

Development of agricultural technologies in developing countries to respond to climate change

Program name

Environment and Natural Resource Management

Term of research

FY 2012 (FY 2005-FY 2012)

Responsible researcher

Kobayashi Nobuya ( Institute of Crop Science, NARO )

KAKEN Researcher No.: 70252799

Fujita Daisuke ( Institute of Crop Science, NARO )

KAKEN Researcher No.: 80721274

Tagle Analiza G. ( International Rice Research Institute )

Ebron Leodegario A. ( International Rice Research Institute )

Fukuta Yoshimichi ( Tropical Agriculture Research Front )

KAKEN Researcher No.: 40399374
MIERUKA ID: 001798

Ishimaru Tsutomu ( Biological Resources and Post-harvest Division )

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Publication, etc.

Fujita et al. (2012), Breeding Science. 62:18-26

Japanese PDF

2012_03_A4_ja.pdf84.24 KB

English PDF

2012_03_A4_en.pdf149.35 KB