Near-isogenic lines carrying QTL for high spikelet number with the genetic background of IR64, an Indica-type rice variety
IR64, recognized globally as a high quality Indica-type rice variety, was first released by the International Rice Research Institute (IRRI) in 1985. To improve the yield potential of IR64, a set of near-isogenic lines (NILs) of IR64 with increased total spikelet number per panicle (TSN) was developed using New Plant Type (NPT) varieties as tropical Japonica-type donor parents.
A total of five NILs derived from different donor parents were developed through marker-assisted selection. Regardless of the donor parents, Quantitative Trait Locus (QTL) for high TSN was detected on common region of the long arm of chromosome 4 (Table 1, Fig. 1). We designated this QTL as qTSN4. Marker information linked to qTSN4 is shown in Table 1. NILs have 196-239 spikelets per panicle (i.e., 40-70% greater TSN than IR64), which is attributed to the increase in larger number of spikelets on the secondary and ternary rachis branches. Variation in TSN was observed among NILs; the number of spikelets was greatest in IR64-NIL2 (IR65564-2-2-3 as donor parent) and in IR64-NIL4 (IR66215-44-2-3 as donor parent).
In this study, we succeeded in developing five NILs for high TSN with a rice variety (IR64) genetic background by employing molecular marker-assisted selection. The materials are expected to be useful for enhancing the yield potential of rice varieties. These NILs are also available for understanding the genetic basis of TSN and the effects of single QTL/gene by testing it under different environmental conditions. Further investigation is required to determine if the variation in panicle architecture is simply due to the allelic effects or not.
Japan International Research Center for Agricultural Sciences Biological Resources and Post-harvest Division
- Research project
- Program name
- Term of research
FY 2012 （FY 2005-FY 2012）
- Responsible researcher
Kobayashi Nobuya ( Institute of Crop Science, NARO )KAKEN Researcher No.: 70252799
Fujita Daisuke ( Institute of Crop Science, NARO )KAKEN Researcher No.: 80721274
Tagle Analiza G. ( International Rice Research Institute )
Ebron Leodegario A. ( International Rice Research Institute )
Fukuta Yoshimichi ( Tropical Agriculture Research Front )
Ishimaru Tsutomu ( Biological Resources and Post-harvest Division )
- Publication, etc.
Fujita et al. (2012), Breeding Science. 62:18-26
- Japanese PDF
- English PDF