Occurrence of male sterile cytoplasm in 'Kurodane Kinugasa' snap bean



'Kurodane Kinugasa' Snap bean produced semi-sterile hybrids when crossed as a seed parent with 'Haibushi'. Pollen stainability of the hybrid plants by aceto-carmine staining was 36.8 %. Since the reciprocal cross, 'Haibushi'×'Kurodane Kinugasa' exhibited a high pollen stainability (83.8 %), the occurrence of male sterility in this cross combination is concluded to be cytoplasmic. B1F1 progeny derived from ('Kurodane Kinugasa'×'Haibushi')×'Kurodane Kinugasa' segregated into semi-sterile (12.8 to 26.5 % pollen stainability) and fertile (87.0 to 95.4 % pollen stainability) plants in a 1:1 ratio. 'Kurodane Kinugasa' is considered to have a single major restorer gene whose action is gametophytic. We have developed a cytoplasmic male sterile line of 'Haibushi' by recurrent backcrossing. 'Haibushi' is a heat-tolerant variety and exhibits high yielding ability in summer cultivation. To establish F1 varieties of snap bean using male-sterile 'Haibushi' line as one parent, we are now exploring snap bean lines with sporophytic restorer gene(s) against this cytoplasmic male sterility.


Japan International Research Center for Agricultural Sciences Okinawa Subtropical Station


Technical A

Term of research

FY2000 (FY1998-2001)

Responsible researcher

EGAWA Yoshinobu ( Okinawa Subtropical Station )

TSUKAGUCHI Tadashi ( Bio-oriented Technology Research Advancement Institution )

TAKEDA Hiroyuki ( Okinawa Subtropical Station )

SUZUKI Katsumi ( Okinawa Subtropical Station )

Japanese PDF

2000_29_A3_ja.pdf845.19 KB

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