Occurrence of male sterile cytoplasm in 'Kurodane Kinugasa' snap bean
Description
[Synopsis]
'Kurodane Kinugasa' Snap bean produced semi-sterile hybrids when crossed as a seed parent with 'Haibushi'. Pollen stainability of the hybrid plants by aceto-carmine staining was 36.8 %. Since the reciprocal cross, 'Haibushi'×'Kurodane Kinugasa' exhibited a high pollen stainability (83.8 %), the occurrence of male sterility in this cross combination is concluded to be cytoplasmic. B1F1 progeny derived from ('Kurodane Kinugasa'×'Haibushi')×'Kurodane Kinugasa' segregated into semi-sterile (12.8 to 26.5 % pollen stainability) and fertile (87.0 to 95.4 % pollen stainability) plants in a 1:1 ratio. 'Kurodane Kinugasa' is considered to have a single major restorer gene whose action is gametophytic. We have developed a cytoplasmic male sterile line of 'Haibushi' by recurrent backcrossing. 'Haibushi' is a heat-tolerant variety and exhibits high yielding ability in summer cultivation. To establish F1 varieties of snap bean using male-sterile 'Haibushi' line as one parent, we are now exploring snap bean lines with sporophytic restorer gene(s) against this cytoplasmic male sterility.
- Affiliation
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Japan International Research Center for Agricultural Sciences Okinawa Subtropical Station
- Classification
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Technical A
- Term of research
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FY2000 (FY1998-2001)
- Responsible researcher
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EGAWA Yoshinobu ( Okinawa Subtropical Station )
TSUKAGUCHI Tadashi ( Bio-oriented Technology Research Advancement Institution )
TAKEDA Hiroyuki ( Okinawa Subtropical Station )
SUZUKI Katsumi ( Okinawa Subtropical Station )
- ほか
- Japanese PDF
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