Simple crossing method of snap bean using physiological pollen sterility caused by high temperature treatment
Description
[Synopsis]
High temperature causes pollen sterility in snap bean ( Phaseolus vulgaris L.) rather easily. When 'Light Green' snap bean was exposed to a high temperature (35 ℃) for 72 hours, the flowers that opened 8 to 12 days after the onset of heat treatment did not set pods by selfing. However, pod setting was 75% and 100 % 10 days and 11 days after the treatment, respectively, when the heat-treated 'Light Green' was crossed with fertile pollen of 'Haibushi' snap bean following the procedures described below. Crossing was carried out on the day of flowering without castration. Stigma easily protruded from the open mouth of keel petal by pressing down wing petals with fingers, which was pollinated with fertile pollen of 'Haibushi'. Hybridity of the plants obtained from the crossing were examined after 8 to 12 days of the heat treatment based on the coloration of hypocotyl. It was concluded that crossing method proposed by us did not require skilful castration technique and enabled us to produce F1 hybrids for practical and experimental uses efficiently and highly successfully.
- Affiliation
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Japan International Research Center for Agricultural Sciences Okinawa Subtropical Station
- Classification
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Technical A
- Term of research
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FY2000 (FY1998-2001)
- Responsible researcher
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EGAWA Yosinobu ( Biological Resources Division )
TSUKAGUCHI Tadashi ( Bio-oriented Technology Research Advancement Institution )
TAKEDA Hiroyuki ( Okinawa Subtropical Station )
SUZUKI Katsumi ( Okinawa Subtropical Station )
- ほか
- Publication, etc.
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- Japanese PDF
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2000_28_A3_ja.pdf784.89 KB