Molecular cloning of Na+ -ATPase cDNA from a marine alga, Heterosigma akashiwo (Sodium-pump in plant cell)
Description
[Synopsis]
Soil salinity is a major abiotic stress in agricultural production worldwide. Breeding of salt tolerant crops through genetic engineering is considered as one of the effective methods to solve that problem. Novel Na+ -ATPase (HANA) cDNA from H. akashiwo was cloned as a target gene for the transformation. The full-length HANA cDNA was 4467-bp long and coded for a 1330-amino acid protein with a molecular weight of 146 and 306. HANA showed around 40% homology with animal Na+ /K+ -ATPase α-subunits in regards to identity of amino acids. A hydrophilic sequence of 285 amino acid residues that showed no homology with any sequence listed in databases existed in the M7-M8 junction of HANA. A phylogenetic tree of a P-type ATPase family comprised of three major clusters, mainly correlating with cation specificity, as follows: Ca2+ -ATPases, H+ -ATPases, and Na+ /K+ -ATPases. HANA is included in the cluster of Na+ /K+ -ATPases, being especially close to the Na+ /K+ -ATPases in invertebrates such as Artemia, Drosophila, or Hydra. However, yeast Na+ -ATPases are classified into the cluster of Ca2+ -ATPases. This is the first report on the primary structure of putative Na + transporting ATPase from the plant cells.
- Affiliation
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Japan International Research Center for Agricultural Sciences Okinawa Subtropical Station
- Classification
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Technical A
- Term of research
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FY2000 (FY1992-2001)
- Responsible researcher
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SHONO Mariko ( Okinawa Subtropical Station )
WADA Masato ( Fruit Tree Research Station )
HARA Yukichi ( Tokyo Medical and Dental University )
- ほか
- Japanese PDF
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