Molecular cloning of Na+ -ATPase cDNA from a marine alga, Heterosigma akashiwo (Sodium-pump in plant cell)

Description

[Synopsis]

Soil salinity is a major abiotic stress in agricultural production worldwide. Breeding of salt tolerant crops through genetic engineering is considered as one of the effective methods to solve that problem. Novel Na+ -ATPase (HANA) cDNA from H. akashiwo was cloned as a target gene for the transformation. The full-length HANA cDNA was 4467-bp long and coded for a 1330-amino acid protein with a molecular weight of 146 and 306. HANA showed around 40% homology with animal Na+ /K+ -ATPase α-subunits in regards to identity of amino acids. A hydrophilic sequence of 285 amino acid residues that showed no homology with any sequence listed in databases existed in the M7-M8 junction of HANA. A phylogenetic tree of a P-type ATPase family comprised of three major clusters, mainly correlating with cation specificity, as follows: Ca2+ -ATPases, H+ -ATPases, and Na+ /K+ -ATPases. HANA is included in the cluster of Na+ /K+ -ATPases, being especially close to the Na+ /K+ -ATPases in invertebrates such as Artemia, Drosophila, or Hydra. However, yeast Na+ -ATPases are classified into the cluster of Ca2+ -ATPases. This is the first report on the primary structure of putative Na + transporting ATPase from the plant cells.

Affiliation

Japan International Research Center for Agricultural Sciences Okinawa Subtropical Station

Classification

Technical A

Term of research

FY2000 (FY1992-2001)

Responsible researcher

SHONO Mariko ( Okinawa Subtropical Station )

WADA Masato ( Fruit Tree Research Station )

HARA Yukichi ( Tokyo Medical and Dental University )

ほか
Japanese PDF

2000_27_A3_ja.pdf943.72 KB

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