JIRCAS developed BNI (Biological Nitrification Inhibition)-enabled wheat to enhance nitrogen use efficiency and reduce environmental impact. BNI suppresses soil nitrification, reducing nitrate leaching and nitrous oxide emissions, offering a sustainable solution to nitrogen-related agricultural challenges. Wheat is vital in Nepal, especially in the Terai (a lowland region), but yields remain low due to limited fertilizer access and reliance on imports. Fertilizer shortages and high costs prevent farmers from achieving optimal yields. BNI-enabled wheat helps narrow the yield gap by improving nitrogen use efficiency, making it suitable for Nepal’s conditions. JIRCAS, in collaboration with local institutions, is testing BNI varieties adapted to Nepal’s agro-ecological zones, aiming to replace existing CIMMYT lines. This initiative supports strengthening food security, reduces environmental burden, and promotes sustainable wheat production. Initial trials in Nepal used “Munal,” but issues with yellow rust shifted the focus to the Terai region, where “Borlaug 2020” was introduced and its BNI-enabled version tested. Nepal plans to adopt and eventually breed its own BNI varieties, supported by international collaboration and research.