Complete nucleotide sequence and genetic organization of papaya leaf distortion mosaic virus RNA
Description
[Synopsis]
Papaya leaf distortion mosaic virus (PLDMV) and Papaya ringspot virus P type (PRSV-P) have been reported as the causal viruses of papaya disease in Japan. Both of them belong to the genus Potyvirus. The complete nucleotide sequence of the RNA genome of PLDMV was determined by overlapping cDNA clones and primer extension. The genomic RNA is 10,153 nucleotides in length, excluding the poly(A) tract, and contains one large open reading frame encoding a polyprotein of 3,269 amino acids. Cleavage sites were predicted by analogy with the other potyviruses. The genetic organization of PLDMV RNA is proposed to be P1, the first proteinase; Hc-Pro, helper component proteinase; P3, the third protein; 6K1 and 6K2, 6kDa protein 1 and 2; CI, cytoplasmic inclusion protein; NIa, nuclear inclusion protein "a" including the VPg (NIa-Vpg) and NIa proteinase (NIa-Pro); NIb, nuclear inclusion protein "b"; CP, coat protein. The genetic organization of PLDMV is similar to that of the other potyviruses. The P1 protein of potyviruses is the most variable and may be considered important for identification of individual potyviruses. It might be useful for distinguishing between PLDMV and PRSV-P by RT-PCR. The sequence will be used for achieving transformation into papaya to make a PLDMV resistant papaya.
- Affiliation
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Japan International Research Center for Agricultural Sciences Okinawa Subtropical Station
- Classification
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Technical A
- Term of research
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FY2000 (FY1999-2001)
- Responsible researcher
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MAOKA Tetsuo ( Okinawa Subtropical Station )
NODA Chiyoichi ( Okinawa Subtropical Station )
- ほか
- Japanese PDF
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2000_26_A3_ja.pdf865.97 KB