Isolation of white-spored mutants from the koji molds for the production of Kecap, an Indonesian soy sauce
Country
Indonesia
Description
[Synopsis]
A collaborative joint research work between JIRCAS and Research Institute for Legume and Tuber Crops (RILET) on improvement of fermented soybean food, Kecap, was conducted at RILET in Indonesia. Koji samples were collected from Kecap and Tauco factories in Java Island. Aspergillus was the dominant mold genus in five out of six koji samples, while one sample contained Mucorales as dominant fungi. Aspergillus contributed to hydrolysis of soybean proteins much more than the Mucoraceous fungus isolated from Tempe starter did. Although no aflatoxin was detected in these koji samples, two isolates out of 24 isolates from the Kecap and Tauco koji samples produced aflatoxins. In order to develop a pure culture starter for Kecap koji making, white-spored mutants were isolated by UV-irradiation from the selected Aspergillus strains. All white-spored mutants were proved to be aflatoxin-negative. The Kecap koji prepared by inoculating the mutant could be distinguished from that prepared by inoculating the original koji mold strain or the aflatoxin producer by developing a typical white color spores during koji making. The formol nitrogen contents of the Kecap mashes prepared by using white-spored mutants were almost same as those of prepared using original strains. Therefore, it is expected that a white-spored mutant can be applied as a starter for Kecap koji making from the standpoint of food safety.
- Affiliation
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Japan International Research Center for Agricultural Sciences Crop Production and Postharvest Technology Division
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National Food Research Institute
Research Institute for Legume and Tuber Crops
- Classification
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Technical A
- Term of research
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FY2000 (FY1998-2000)
- Responsible researcher
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NIKKUNI Sayuki ( Crop Production and Postharvest Technology Division )
GOTO Tetsuhisa ( National Food Research Institute )
GINTING Erliana ( Research Institute for Legume and Tuber Crops )
ANTARLINA Sri S. ( Research Institute for Legume and Tuber Crops )
UTOMO Joko S. ( Research Institute for Legume and Tuber Crops )
- ほか
- Japanese PDF
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2000_10_A3_ja.pdf711.87 KB