In situ hybridization to detect bovine cytokine mRNAs in protozoan disease
Description
[Synopsis]
Cytokines are a family of low molecular weight protein regulatory molecules involved in inflammation and immunity. They play an important role in the immune system to control cell growth and differentiation of immune‐competent cells. An in situ hybridization protocol using non‐radioactively labeled RNA probes was established to detect bovine cytokine mRNAs （TNF-α, IFN-γ, IL-2, IL-4, IL-6, IL-10 and IL-12p35） on cytospin preparations.
- Affiliation
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Japan International Research Center for Agricultural Sciences Animal Production and Grassland Division
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International Livestock Research Institute
- Classification
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Technical A
- Term of research
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FY1997 (FY1994-1997)
- Responsible researcher
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MATSUBARA Yutaka ( Animal Production and Grassland Division )
- ほか
- Japanese PDF
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1997_13_A3_ja.pdf1.21 MB