LA-PCR method for mitochondrial DNA RFLP analysis in pigs and wild pigs

Description

[Synopsis]

We developed a simple method for amplification of pig and wild pig mitochondrial DNA (mtDNA), 15.2kb, and for mtDNA RFLP analysis using the long-Accurate (LA) PCR method. The usefulness of the present method for mtDNA amplification and mtDNA RFLP analysis was confirmed in Luzon native pigs and Luzon wild pigs.

Affiliation

Japan International Research Center for Agricultural Sciences Animal Production and Grassland Division

National Institute of Animal Industry

University of the Philippines

Classification

Technical A

Term of research

FY1995(FY1995)

Responsible researcher

KOMATSU Masanori ( Animal Production and Grassland Division )

MATIAS Job M. ( University of the Philippines )

TAKEDA Kumiko ( National Institute of Animal Industry )

ONISHI Akira ( National Institute of Animal Industry )

ほか
Publication, etc.

Japanese PDF

1995_13_A3_ja.pdf1.33 MB

Related Research Highlights