Gene discovery of SPIKE -A unique gene from a rice landrace increases grain yield of Indica-type cultivars-

Description

Increasing crop production is essential for securing the future food supply in developing countries. Total spikelet number per panicle (TSN) is one of the most important traits to increase grain productivity in rice (Oryza sativa L.). We previously reported the detection of qTSN4, a QTL for increasing TSN on the long arm of chromosome 4 derived from new plant type (NPT) cultivars with the genetic background of IR64 (Refer the Research Highlight in 2012). In this study, we attempted to clone the gene for qTSN4. We further conducted characterization of agronomic traits of a near isogenic line (NIL) with the gene. To understand the effect of the genein different genetic backgrounds, we introduced it into six indica cultivars popular in South and Southeast Asian countries by marker assisted selection.

We successfully identified a causative gene for qTSN4, designated here as SPIKE (SPIKELET NUMBER) by map based cloning using 7996 BC4F3 plants of an NPT cultivar as a donor and IR64 as a recurrent parent (Fig. 1A). NIL for SPIKE had higher TSN (Fig. 1B), wider flag leaf (Fig. 1C) and heavier root dry weight (Fig. 1D) than those of IR64. Rate of filled grain were also higher, but panicle number per plant and 1000-grain weight were lower in the NIL (data not shown). Notably, the grain appearance of the NIL was significantly improved (Fig. 2A), presumably owing to a strengthening of assimilate supply to the larger number of spikelets by an increase in vascular bundle number (Fig. 2B). Consequently, the grain yield of the NIL was consistently higher by 13-36% than that of IR64 over four cropping seasons, significantly so in three of the four seasons (Fig. 2C). SPIKE also increased TSN in six cultivars popular in South and Southeast Asia (Fig. 3), confirming its effectiveness in various genetic backgrounds.

SPIKE would be a unique gene to increase grain yields of indica cultivars in South and Southeast Asia through molecular marker-assisted breeding, thus contributing to food security in these regions.

Figure, table

  1. Fig. 1 Chromosomal location of SPIKE (A), panicle architecture (B), flag leaf width (C) and root dry weight (D). Values are mean ± SD. **Significant at 1% level by t-test.

    Fig. 1 Chromosomal location of SPIKE (A), panicle architecture (B), flag leaf width (C) and root dry weight (D). Values are mean ± SD. **Significant at 1% level by t-test.

  2. Fig. 2 Percentages of grain chalkiness (A), number of vascular bundle at panicle neck (B) and grain yield (C). Values are mean ± SD (A, B) or SE (C). Significant at ***0.1%, **1% and *5% level by t-test.

    Fig. 2 Percentages of grain chalkiness (A), number of vascular bundle at panicle neck (B) and grain yield (C). Values are mean ± SD (A, B) or SE (C). Significant at ***0.1%, **1% and *5% level by t-test.

  3. Fig. 3 Total spikelet number per panicle between Indica-type cultivars with and without SPIKE. Values are mean ± SE. Significant at ***0.1%, **1% and *5% level by t-test.

    Fig. 3 Total spikelet number per panicle between Indica-type cultivars with and without SPIKE. Values are mean ± SE. Significant at ***0.1%, **1% and *5% level by t-test.

Affiliation

Japan International Research Center for Agricultural Sciences Biological Resources and Post-harvest Division

Classification

Administration A

Country

Indonesia

Research project

Development of agricultural technologies in developing countries to respond to climate change

Program name

Environment and Natural Resource Management

Term of research

FY 2013 (FY 2005-FY 2013)

Responsible researcher

Kobayashi Nobuya ( Institute of Crop Science, NARO )

KAKEN Researcher No.: 70252799

Fujita Daisuke ( Institute of Crop Science, NARO )

KAKEN Researcher No.: 80721274

Tagle Analiza G. ( International Rice Research Institute )

Koide Yohei ( Research Fellow of the Japan Society for the Promotion of Science )

KAKEN Researcher No.: 70712008

Sasaki Kazuhiro ( University of Tokyo )

KAKEN Researcher No.: 70513688

Gannaban Ritchel B. ( International Rice Research Institute )

Fukuta Yoshimichi ( Tropical Agriculture Research Front )

KAKEN Researcher No.: 40399374
MIERUKA ID: 001798

Ishimaru Tsutomu ( Biological Resources and Post-harvest Division )

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Publication, etc.

Fujita, D. et al. (2013) PNAS, 110: 20431-20436

https://doi.org/10.1073/pnas.1310790110

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