Determination of amino acid sequence and site of mRNA expression of four vitellins in the giant freshwater prawn, Macrobrachium rosenbergii
Description
[Synopsis]
Four major yolk proteins, designated as vitellins (Vns) Macr-VnA, B, C and D, were extracted from mature ovary of Macrobrachium rosenbergii. These were purified to homogeneity by reversed-phase HPLC employing a unique separation system based on the hydrophobic properties of the Vn molecule. More than 33 N-terminal and 57 internal amino acid residues were determined for each of the four Vns. The cDNA fragments encoding the four Vns were amplified by PCR using degenerate oligonucleotide primers derived from the N-terminal and internal amino acid sequences. These cDNA fragments were cloned, sequenced and used as probes to examine the transcription of mRNAs encoding the four Vns. Significant accumulation of these mRNAs was observed in female hepatopancreas only, while mRNA expression was not detected in male hepatopancreas or any other female tissue including ovary, subepidermal adipose tissue, gill and muscle.
- Affiliation
-
Japan International Research Center for Agricultural Sciences Fisheries Division
- Classification
-
Technical A
- Term of research
-
FY1999-2000
- Responsible researcher
-
WILDER Marcy Nicole ( Fisheries Division )
YANG Wei-Jun ( Fisheries Division )
- ほか
- Japanese PDF
-
1999_12_A3_ja.pdf813.68 KB