Isolation of Protoplasts from Suspension Culture and Subsequent Shoot Regeneration in Sugarcane
ISSN | 00213551 |
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NII recode ID (NCID) | AA0068709X |
Cell suspension cultures with an ability to regenerate plants were initiated from 10 sugarcane clones tested. Cultures were initiated and maintained in modified N6 liquid medium containing 2 mg/l 2,4-D, 500 mg/l casein hydrolysate and 3% sucrose. Protoplasts isolated from these suspensions were embedded in 1.2% agarose (modified KM8P medium) and cultured in modified KM8P medium with the addition of nurse culture cells from suspension culture. After 5 to 8 weeks of culture, calluses were obtained in 5 clones. Calluses derived from protoplasts could not form any organs in the following culture on R2 regeneration medium. Only, protoplastderived calluses of NiF4 (cultivar) cultured for 2 weeks on PR4 medium regenerated green shoots on R9 medium. There shoots could not develop to plant, and died in the following culture.
Date of issued | |
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Creator |
Makoto MATSUOKA
Takayoshi TERAUCHI
Makoto KOBAYASHI
Moriyuki SHODA Hiroshi NAKANO |
Available Online | |
NII resource type vocabulary | Journal Article |
Volume | 30 |
Issue | 1 |
spage | 21 |
epage | 26 |
Language | eng |