Plant Regeneration from Protoplasts of Satsuma Mandarin(Citrus unshiu Marc.) via Somatic Embryogenesis

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text

Protoplasts were isolated from the embryogenic calli of satsuma mandarin (Citrus unshiu Marc.) cv. Tokumori-Wase and cultured in the Gellan Gum-embedded Murashige and Tucker medium containing 40 mg/l adenine, 0.15M sucrose and 0.45M mannitol. The percentage of colony formation (plating efficiency) in 40 days of culture was 46%, which was much higher than that in MT medium without adenine. Most of the colonies grew to the size of about 1 mm in 2 months of culture and they were successfully transferred onto the MT medium containing 5% lactose for embryoid formation. Five percent of these embryoids germinated normally and grew to plantlets in 30-60 days after transplanting to the MT medium containing 1 mg/l gibberellic acid (GA3), 3% sucrose and 0.2% Gellan Gum. Protoplasts obtained in the culture of other two selections, Miyagawa-Wase and Okitsu-Wase also regenerated plants by using the same method as above.

Date of issued
Creator Hisato KUNITAKE Hiroshi KAGAMI Masahiro MII
Available Online
NII resource type vocabulary Journal Article
Volume 24
Issue 4
spage 287
epage 291
Language eng

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