Manufacturing method of functional foods that has alpha-glucosidase inhibitory activity using Bacillus amyloliquefaciens

Description

The demand for functional foods has also increased in developing countries because of the rise in health concerns. Therefore, we performed screening of functional substances from the same species of bacteria that had been isolated from fermented foods in East and Southeast Asia. As a result, Bacillus amyloliquefaciens that had been isolated from a Thai fermented food, Thua nao, and similar foods, was found to produce alpha-glucosidase inhibitory substances. Therefore, we developed a manufacturing method for functional foods that had alpha-glucosidase inhibitory activity using B. amyloliquefaciens.
  B. amyloliquefaciens strains, NBRC3022, NBRC14141, NBRC15535, and Bacillus subtilis NBRC13719 were obtained from the Biological Resource Center, National Institute of Technology and Evaluation, Japan. Commercial natto bacterium was isolated from a natto purchased from a Japanese market. Rice, wheat, corn and soybean were fermented by these bacteria (Fig. 1). Fermentation extracts of B. amyloliquefaciens showed alpha-glucosidase inhibitory activity, while those of B. subtilis and commercial natto bacterium showed no activity (Fig. 2). Alpha-glucosidase inhibitory activity of the culture extracts using LB media was also similar to that of the fermentation extracts (Fig. 2).
  These results showed that newly functional food materials with alpha-glucosidase inhibitory activity could be produced using the bacteria, B. amyloliquefaciens. These food materials may be utilizable for the manufacture of nutrition foods with inhibitory effect on elevated blood sugar levels.

Figure, table

  1.  

    図1 種々のB. amyloliquefaciensにより製造した大豆発酵物
    Fig. 1. Soybeans fermented with several strains of B. amyloliquefaciens.
  2.  

    Fig. 2. The alpha-glucosidase inhibitory activities of fermented foods and culture extracts.
    Fig. 2. The alpha-glucosidase inhibitory activities of fermented foods and culture extracts.
    The vertical axis indicates alpha-glucosidase relative activity (%). The value of 100% means alpha-glucosidase activity without the presence of any inhibitor.
Affiliation

Japan International Research Center for Agricultural Sciences Post-harvest Science and Technology Division

Classification

Research B

Term of research

FY2006~2010

Responsible researcher

NIRASAWA Satoru ( Post-harvest Science and Technology Division )

YOSHIHASHI Tadashi ( Post-harvest Science and Technology Division )

LI Lite ( China Agricultral University )

MORI Yutaka ( Post-harvest Science and Technology Division )

ほか
Publication, etc.

Nirasawa, et al. Japanese Patent Application No. 2010-206162

Japanese PDF

2010_seikajouhou_A4_ja_Part14.pdf135.14 KB