Distinction of sucrose phosphate synthase loci in sugarcane
Description
[Synopsis]
Sucrose phosphate synthase (SPS) is considered to be an important enzyme in sucrose accumulation in sugarcane stems, as well as in sucrose transportation in photosynthesis of higher plants. Sugarcane genomic DNA was analyzed by southern blot analysis using combination of three restriction enzymes and four DNA fragments constructed from maize SPS cDNA as probes. Two recognition sites of each Hind III and Sac I enzyme commonly existed in detected sugarcane SPS gene. Southern blot analysis using combination of Sac I enzyme and the 3'-end-side fragment of maize SPS cDNA as probe is considered to indicate SPS loci variation in sugarcane. Polymorphic band patterns were observed in sugarcane genetic resources by this method. The method enables SPS loci screening on genetic resources.
- Affiliation
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Japan International Research Center for Agricultural Sciences Okinawa Subtropical Station
- Classification
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Technical A
- Term of research
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FY2000 (FY1997-1999)
- Responsible researcher
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TERAUCHI Takayoshi ( Okinawa Subtropical Station )
SAITOU Akira ( Kyushu Agricultural Experiment Station )
MIYAZAKI Chikara ( Japan Science and Technology Corporation )
KIMURA Takashi ( Kyushu Agricultural Experiment Station )
IDETA Osamu ( Okinawa Subtropical Station )
MATSUOKA Makoto ( Okinawa Subtropical Station )
- ほか
- Japanese PDF
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2000_30_A3_ja.pdf774.48 KB