Japan International Research Center for Agricultural Sciences | JIRCAS

Gamma-glutamylcysteine Synthetase Gene Homolog (gshA) is Important in Glutathione Homeostasis in Aspergillus oryzae

Japan Agricultural Research Quarterly
ISSN
00213551
NII recode ID (NCID)
AA0068709X
Full text

Aspergillus oryzae is a filamentous fungus used for the manufacture of medicines and fermented foods. Glutathione is a tripeptide contained in variety of organisms and is involved in tolerance to various stresses. A. oryzae has a putative γ-glutamylcysteine synthetase gene (gshA) in its genome. To investigate the effect of the gene expression level of gshA on the amount of glutathione in A. oryzae, we generated a transformant where gshA expression was regulated by the thiamine-repressible thiA promoter. When the transformant was cultured on CzapekDox (CD) agar with added thiamine, the intracellular level of glutathione in the transformant decreased in a dose-dependent manner. Results of this study discovered that in oxidative stress conditions, repression of gshA gene expression with thiamine caused inhibition of growth of the transformant. Also, it was found that the gshA gene plays an important role in glutathione homeostasis in A. oryzae, and this gene may also be necessary for oxidative stress response.

Date of issued2018-10-01
CreatorRyota HATTORISawaki TADAMayumi MATSUSHITA-MORITASatoshi SUZUKIKen-Ichi KUSUMOTO
Subjectglutathione synthesisgrowthoxidative stress
PublisherJapan International Research Center for Agricultural Sciences
Dates2018-10-09
NII resource type vocabularyJournal Article
Volume52
Issue4
spage301
epage305
DOI10.6090/jarq.52.301
Languageeng