Nucleotide Sequences of Porcine α1 and α2 Chains of Type I Collagen cDNA and Their Different Expression Levels in Tissues

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Nucleotide Sequences of Porcine α1 and α2  Chains  of  Type I Collagen cDNA and Their Different Expression Levels  in  Tissues
Full text

To understand the role of type I collagen in pig health and pork production, we report the complete coding sequences of porcine COL1A1 and COL1A2 cDNAs as a first step toward the functional analysis of these genes. The coding sequences of cDNA showed high similarity with those of other mammals (88.6%-95.0% and 86.8%-93.3%, respectively). The deduced amino acid sequences of the porcine COL1A1 and COL1A2 mature proteins showed 91.3%-97.1% and 89.5%-96.0% identity with one of the other mammalian sequences, respectively. Expression analysis showed that these genes were more highly expressed in connective tissues such as skin and back fat than in other tissues. The expression ratio of these two genes is similar in almost all porcine tissues examined, although the COL1A1 gene in back fat and muscle, and the COL1A2 gene in testis are more highly expressed than the other gene.

 

Date of issued
Creator Satoshi YANAI Toshimi MATSUMOTO Etsumori HARADA Eiji KOBAYASH
Subject cDNA sequence expression pattern
Publisher Japan International Research Center for Agricultural Sciences
Available Online
NII resource type vocabulary Journal Article
Volume 52
Issue 2
spage 149
epage 154
DOI 10.6090/jarq.52.149
Language eng

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