Distribution of Two Distinct Genotypes of Citrus Greening Organism in the Ryukyu Islands of Japan

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text
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The Asian type “Candidatus Liberibacter asiaticus” (Las, citrus greening organism) is severely damaging citrus production in Asia including Japan. Our previous study suggested that the bacteriophage-type DNA polymerase region (DNA pol) would be useful for molecular differentiation in different Southeast Asian Las isolates. Moreover, Las isolates originated from most of the Southeast Asian regions harbor the DNA pol gene, whereas Japanese ones lack this region. These preliminary findings lead us to a hypothesis that all Japanese isolates lack DNA pol. To try this hypothesis, we collected citrus leaf samples infected with Las throughout the Ryukyu Islands, and examined them by a duplex PCR that could simultaneously amplify two DNA fragments of DNA pol and nusG-rplK operon of Las. The duplex PCR was applied to the collection of 65 Las isolates. Both DNA pol and nusG-rplK operon were successfully amplified from nine isolates, whereas only nusG-rplK operon was amplified with the other 56 isolates. These nine isolates with DNA pol originated from the Hateruma, Irabu, Kohama, Miyako, Tarama, and Yonaguni Islands, which are geographically close to Taiwan. The nucleotide sequence of DNA pol of these nine isolates was identical, and was also the same as four Taiwanese isolates reported previously. These results suggest that Japanese Las isolates comprise at least two distinct genotypes, and the genotype that had DNA pol is highly homogeneous.

Date of issued
Creator TOMIMURA Kenta FURUYA Noriko MIYATA Shin-ichi HAMASHIMA Akiko TORIGOE Hiroaki MURAYAMA Yuko KAWANO Shinji OKUDA Mitsuru SUBANDIYAH Siti SU Hong-Ji IWANAMI Toru
Subject

citrus greening disease

Diaphorina citri

duplex PCR

Huanglongbing

Publisher Japan International Research Center for Agricultural Sciences
Available Online
NII resource type vocabulary Journal Article
Volume 44
Issue 2
spage 151
epage 158
DOI 10.6090/jarq.44.151
Rights Japan International Research Center for Agricultural Sciences
Language eng

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