Skim Milk Drastically Improves the Efficacy of DNA Extraction from Andisol, a Volcanic Ash Soil

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text
39-04-04.pdf241.26 KB

There is an increasing interest in the extraction of DNA from soil, since molecular techniques facilitate the analysis of the microbial community including unculturable microorganisms. Though various methods of direct DNA extraction have been devised, it still remains difficult to extract DNA from some soils. We developed an improved method to extract DNA from such “recalcitrant soils”. Andisol, volcanic ash soils are recalcitrant in this context. Skim milk increased the efficacy of DNA extraction from such soils, when added to the extraction buffer as an adsorption competitor to soil particles. This method is applicable to molecular community analysis of soils which strongly adsorb DNA.

Date of issued
Creator TAKADA HOSHINO Yuko MATSUMOTO Naoyuki
Subject

environmental DNA

microbial community analysis

molecular methods

unculturable microorganisms

Publisher Japan International Research Center for Agricultural Sciences
Available Online
NII resource type vocabulary Journal Article
Volume 39
Issue 4
spage 247
epage 252
DOI 10.6090/jarq.39.247
Rights Japan International Research Center for Agricultural Sciences
Language eng

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