Japan International Research Center for Agricultural Sciences | JIRCAS

Development of an Automatic Silkworm Larvae Hemolymph Collection System by Infrared Laser Beam Incision Technique

Japan Agricultural Research Quarterly
ISSN
00213551
NII recode ID (NCID)
AA0068709X
Full text

To establish an insect factory for mass production of useful substances using silkworms, the author developed a silkworm hemolymph collection system to efficiently collect hemolymph from 5th-instar larvae of silkworms, Bombyx mori. The system automatically performs the serial processes of feeding silkworm larvae to the system, incision, collection of hemolymph, and removal of silkworms after hemolymph collection. This system consists primarily of a silkworm feeder, a conveyer chain with holders for the transport of silkworms, a CO2 laser for infrared laser beam incision of silkworms, a container for hemolymph collection, and two coolers. The hemolymph collection from 4 and 5-day 5th-instar silkworm larvae anesthetized at low temperature could be collected continuously in less than 2.5 min per larva, giving a good prospect for a one-man operation. The amount of hemolymph was 12-13% of the silkworm larva body weight.

Date of issued2004-10-01
CreatorKOBAYASHI Toru
Subject

insect factory

baculovirus

NPV

PublisherJapan International Research Center for Agricultural Sciences
Dates2004-10-01
NII resource type vocabularyJournal Article
Volume38
Issue4
spage235
epage240
DOI10.6090/jarq.38.235
RightsJapan International Research Center for Agricultural Sciences
Languageeng