Digoxigenin (DIG)-Based AFLP Analysis of Three Citrus Cultivars

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text
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A digoxigenin (DIG)-based amplified fragment length polymorphism (AFLP) method was developed to distinguish three closely related citrus cultivars, ‘Kishu’, ‘Mukaku-Kishu’ and ‘Hira-Kishu’. In particular, ‘Mukaku-Kishu’ characterized by seedless fruit is very important for breeding purposes. Initial attempts at using the DIG-based RAPD procedure failed to detect ‘Mukaku-Kishu’-specific bands. However, the DIG-based AFLP method enabled to detect such bands, indicating that this method is more sensitive than the DIG-based RAPD procedure. The detailed protocol for the DIG-based AFLP methods is presented in this paper.

Date of issued
Creator HANADA Hiromi KAYANO Toshiaki KOGA-BAN Yasunori TANAKA Hiroshi TABEI Yutaka




Publisher Japan International Research Center for Agricultural Sciences
Available Online
NII resource type vocabulary Journal Article
Volume 37
Issue 4
spage 225
epage 228
DOI 10.6090/jarq.37.225
Rights Japan International Research Center for Agricultural Sciences
Language eng

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