Specific Amplification of Microsporidian DNA Fragments Using Multiprimer PCR

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text

Using a mixture of several primers, the suitability of multiprimer PCR for the early and simultaneous detection of various kinds of infectious microsporidia of silkworms was evaluated. As a result, specific DNA sequences were amplified by PCR using several primers designed for this study only when genomic DNA of the target microsporidia was used as the DNA template. However, no PCR products were obtained when genomic DNA of the silkworms or other microorganisms was used as the DNA template. In addition, specific DNA sequences were amplified by multiprimer PCR even when silkworms were infected with various kinds of microsporidia. When genomic DNA extracted from silkworm eggs infected with Nosema bombycis was used as the DNA template, the specific DNA sequences were amplified by multiprimer PCR. In addition, similar results were obtained even when genomic DNA extracted from silkworms infected with N. bombycis was used as the DNA template. These findings suggest that multiprimer PCR using several primers designed for this study is suitable for pebrine inspection of silkworm eggs.

Date of issued
Creator HATAKEYAMA Yoshinori HAYASAKA Shoji
Subject

Nosema

Bombyx mori

Silkworm pebrine

transovum transmission

Publisher Japan International Research Center for Agricultural Sciences
Available Online
NII resource type vocabulary Journal Article
Volume 36
Issue 2
spage 97
epage 102
DOI 10.6090/jarq.36.97
Rights Japan International Research Center for Agricultural Sciences
Language eng

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