Sequence Analysis and GTP-Binding Ability of the Minor Core Protein P5 of Rice Gall Dwarf Virus
ISSN | 00213551 |
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NII recode ID (NCID) | AA0068709X |
Analysis of the nucleotide sequence of the genome segment S5 of Rice gall dwarf virus (RGDV), which belongs to the genus Phytoreovirus, revealed that this segment encodes a putative protein of approximately 91 kDa. Antiserum raised against the protein reacted with a minor structural protein of similar size in RGDV, demonstrating that S5 encodes the structural protein of RGDV. The protein named P5 appeared to be a component of viral core particles. Although no overall homology was found to any other proteins, including those of animal-infecting reoviruses, P5 of RGDV exhibited a significant homology to P5 of Rice dwarf virus (50%), which might be a guanylyltransferase, and to P5 of Wound tumor virus (55%). These results, together with the observation that P5 bound guanosine triphosphate suggest that P5 of RGDV shows a guanylyltransferase activity and, moreover, that plant-infecting reoviruses have a similar functional and/or structural organization to that of animal-infecting reoviruses.
Date of issued | |
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Creator | ICHIMI Kenzaburo KIKUCHI Akira MORIYASU Yusuke ZHONG Boxiong HAGIWARA Kyoji KAMIUNTEN Hiroshi OMURA Toshihiro |
Subject |
RGDV Phytoreovirus |
Publisher | Japan International Research Center for Agricultural Sciences |
Available Online | |
NII resource type vocabulary | Journal Article |
Volume | 36 |
Issue | 2 |
spage | 83 |
epage | 87 |
DOI | 10.6090/jarq.36.83 |
Rights | Japan International Research Center for Agricultural Sciences |
Language | eng |